I would either bring the fleece pants and jacket or the long johns, not both. You are in Alabama, it isn't that cold.

If you are only going for one night, leave the toothbrush, toothpaste, soap and towel at home. In fact, bring a small baggie of baby wipes, and skip the toilet paper. Now you have soap and toilet paper .

That is a lot of food for an overnight. Eat a big meal before you leave. Generally, I recommend no-cook foods for the first couple over-nighters. It simplifies life. You can then ditch the stove and pot and not worry about it. There are a lot of other things you can spend your time worrying about getting right before you need to master backpack cooking. I am not saying you have to, just recommending it.

In the end, don't stress it too much. Throw it all in your pack and go. Come back (with pictures) and ask what you could change, or what you didn't like.